Rapid Mouse Brain Imaging Service
We provide mouse brain preparation including tissue clearing and sectioning, followed by VISoR 3D fluorescence imaging. Final deliverables include maximum intensity projections. We also offer PB-level data storage, management, and analysis (stitching, reconstruction, registration, and morphology) via high-performance clusters and proprietary algorithms.
Supported labeling methods: transgenic labeling, viral tracing, CTb tracing, cell nucleus (DAPI) staining, immunofluorescence staining, Nissl staining, etc.
Total Process Duration: 10 Days
Preparation process
1. Sample labeling.Supports various labeling methods including transgenic labeling, viral tracing, CTb tracing, cell nucleus (DAPI) staining, immunofluorescence staining, Nissl staining, etc.
2. Brain extraction and delivery.Send the perfused mouse brain samples to us.
3. Fixation and embedding.Embedded in hydrogel, fixing the sample morphology, not easy to deform.
4. Sectioning into thick slices.Use a vibrating microtome to cut the embedded mouse brain into 300-micron thick slices.
5. Sectioning.Attach 40-50 prepared mouse brain slices to a special slide for clearing
6. Tissue clearing.Use the PuClear method for tissue clearing. If small molecules or antibody labeling is needed, it should be done after clearing.
7. VISoR imaging.Using the VISoR microscope to complete the 3D fluorescence imaging of the entire mouse brain within half an hour at a resolution of 1×1×2.5μm³.
8. 2D reconstruction.Generate maximum intensity projection images of these brain slices for quick assessment of sample research value.
9. More analysis.3D reconstruction, cell counting, neuron morphology reconstruction, etc.
Sample labeling
Supports various labeling methods including transgenic labeling, viral tracing, CTb tracing, cell nucleus (DAPI) staining, immunofluorescence staining, Nissl staining, etc.
Brain extraction and delivery
Send the perfused mouse brain samples to us
Fixation and embedding
Embedded in hydrogel, fixing the sample morphology, not easy to deform
Cut into thick slices
Use a vibrating microtome to cut the embedded mouse brain into 300-micron thick slices
Patch
Attach 40-50 prepared mouse brain slices to a special slide for clearing
Tissue clearing
Use the PuClear method for tissue clearing. If small molecules or antibody labeling is needed, it should be done after clearing.
VISoR imaging
Using the VISoR microscope to complete the 3D fluorescence imaging of the entire mouse brain within half an hour at a resolution of 1×1×2.5μm³.
2D reconstruction
Generate maximum intensity projection images of these brain slices for quick assessment of sample research value
More analyses
3D reconstruction, cell counting, neuron morphology reconstruction, etc.